To investigate whether the glioma stem cells harbor NF1 and TP53 mutations, we have searched the mutation profile of glioma stem cells from two databases, including COSMIC Cell Lines Project (https://cancer.sanger.ac.uk/cell_lines/) and GSE23806 (download from GEO website, https://www.ncbi.nlm.nih.gov/geo/).44 This evidence concerns the gene NF1 and central nervous system cancer.