Amplicon #161 (FBDHS group), an enhancer candidate that overlaps both a DNaseI hypersensitive site in fetal human brain and a copy number variant region near the autism- and epilepsy-associated gene SCN2A, and that displayed particularly strong activity in the screen (Figure 3—figure supplement 1A), showed consistent expression of EGFP in the mouse brain at P7 (Figure 3A, top row). The gene discussed is SCN2A; the disease is autism.