Correlation analysis was performed between the concentration of NFL, neuron-specific enolase (NSE), S100 beta (S100B) and claudin-5 3 hours post-traumatic brain injury (TBI) and ethanol TBI, 4 treatment groups were used: saline–sham (SS), ethanol–sham (ES), saline–TBI (ST) and ethanol–TBI (ET). The gene discussed is CLDN5; the disease is brain injury.