Compared with the control group, the cell viability of nasopharyngeal carcinoma cells in the overexpression group was significantly reduced at 48 h and 72 h (∗p < 0.05, ∗p < 0.05), and the cell viability of nasopharyngeal carcinoma cells in the siRNA group was significantly increased (#p < 0.05, #p < 0.05), indicating that the ability of S100A8/S100A9 to promote the proliferation of nasopharyngeal carcinoma cells is weakened after inhibiting PI3K/Akt pathway activation. The gene discussed is S100A9; the disease is nasopharyngeal carcinoma.