The early stage of NAFLD rats showed higher serum concentration of NEFA (p = 0.0015), TG (p = 0.041), LDL (p = 0.0029), TCHO (p = 0.066), ALP (p = 0.0059), Insulin (p = 0.017), and glucagon (p = 0.026). The gene discussed is GCG; the disease is metabolic dysfunction-associated steatotic liver disease.