We also evaluated the protein amount of a set of 12 cytokines and chemokines, including IL-2, IL-4, IL-5, IL-6, IL-10, IL-12, IL-17A, TNF-α, IFN-γ, MCP-1, MIP-1α, and eotaxin in TIF and plasma samples of C57BL6 mice, engrafted or not engrafted with B16 melanoma cells. The gene discussed is IL10; the disease is melanoma.