To study the role of BRCC3 in the biological behaviors, we created wild type BRCC3 (BRCC3 WT) overexpression and BRCC3 deubiquitinating enzyme-null mutant (H122Q, BRCC3 HQ) overexpression UMUC3 bladder cancer cell lines via lentiviral transfection. Here, BRCC3 is linked to urinary bladder carcinoma.