In the present study, high expression of MKP1 was confirmed in tumor tissues collected from NSCLC patients, and overexpression of MKP1 in H1299 and Calu-3 cells increased glycolysis and proliferation of H1299 and Calu-3 cells that were initially suppressed by si-circ_0006349. The gene discussed is DUSP1; the disease is non-small cell lung carcinoma.