Wang et al. (2011) showed that TPM1 is a validated target of microRNA-21, which negatively regulates its posttranslational expression and leads to arteriosclerosis obliterans. They also showed that overexpression of TPM1 decreased proliferation and migration of hASMCs, whereas its silencing significantly attenuated the antiproliferative and anti-migratory roles of the miR-21 inhibitor (Wang et al., 2011). Our results indicate that CRISPR-based activation of TPM1 expression results in inhibition of the inflammatory response of pHCASMCs and exerts antiproliferative and anti-migratory activity. This evidence concerns the gene TPM1 and arteriosclerosis obliterans.