Currently, subcultures of the cells devoted a cell line named MISB10, which have undergone an excess of 80 passages; they show continuous growth and can recover from repeated cryopreservation cycles; they show responsiveness to EGFR-TKi targeted therapies and form multicellular organotypic spheroids while grown in human tumor microenvironment mimicking 3D culture conditions (33) (Supplementary Figure S6). The gene discussed is EGFR; the disease is neoplasm.