We hypothesized that the infiltration of large numbers of CD8+ T lymphocytes may promote the release of certain signals from the cell surface to recruit other upregulated immune cells, including CD4+ T cells, macrophages, neutrophils, and dendritic cells, thereby modulating the interaction between tumor cells and immune cells to regulate the immunotherapy response, which explained the simultaneous aggregation of several immune cells in our results. Here, CD4 is linked to neoplasm.