To further verify the ability of Gimap5 to regulate lung cancer metastasis, we studied the effect of Gimap5 on the expression levels of EMT biomarkers (E-cadherin, N-cadherin, Snail, Slug, Twist, ZO-1, Vimentin) in PC9, A549 and 1299 cells. This evidence concerns the gene TWIST1 and lung cancer.