Univariate analysis revealed that, clinical parameters of age, preoperative biliary drainage (PBD), CA19-9, TNM stage, tumor size, tumor differentiation, vascular anastomosis method, mode of operation, positive rate of incisal margin, lymph node metastasis, vascular invasion were all obvious associated both with DFS and OS (Table 2), however, gender, preoperative TB, DB, ALT, γGGT, ALP, Glu, amylase, history of smoking, history of diabetes and tumor location were not significantly correlated with OS and DFS (P > 0.05). This evidence concerns the gene GPT and neoplasm.