To assess the impacts of LINC00355 on the oncogenic behaviors of glioma cells, we first synthesized siRNAs against LINC00355 (si-LINC00355#1, si-LINC00355#2, and si-LINC00355#3) and subsequently transfected these LINC00355 siRNAs into U251 and T98G cells because LINC00355 expression was relatively higher in U251 and T98G cells than other glioma cells. This evidence concerns the gene LINC00355 and glioma.