Figure 3(b) shows that DiAcSpm treatment increased the S phase population with a concomitant decrease in the G1 phase population in both SW480 and Caco-2 cells. Accordingly, DiAcSpm upregulated protein expression of the cell cycle regulators, cyclin D1 and cyclin E (Figure 3(c)). ATP is essential for cancer cell survival and malignant growth. We, therefore, determined if DiAcSpm regulated energy production in CRC cells.  Figure 3(d) shows that DiAcSpm treatment increased the energy (ATP) production in both SW480 and Caco-2 cell lines. The gene discussed is CCNE1; the disease is colorectal carcinoma.