WNT5A and colorectal carcinoma: Findings of one study showed that the inhibitory effect of 5-FU on siRNA-induced knockdown Wnt5a expression in unmethylated LoVo and SW480 cells is higher than overexpressed Wnt5a in hypermethylated HCT116 and SW620 cells, suggesting that loss of expression of Wnt5a can have a suppressive effect of 5-FU in CRC cells [46].