WNT5A and colonic neoplasm: To determine the inhibitory effect of Wnt5a on primary colon cancer, the ectopic expression model indicated that overexpression of Wnt5a induced by the ectopic model inhibits cell proliferation and metastasis via suppression of epithelial-mesenchymal transition (EMT), canonical Wnt, and overactivation of Wnt/Ca2+ signaling in HCT116 cell line [45].