From the IHC staining (Figures 4A,B), qRT-PCR (Figure 4C), and western blot (Figures 4D,E), the activation markers, including α-SMA, vimentin, Col I, and Col III, were up-regulated in the livers of CCl4-induced liver fibrosis mice than that in the control, which were further enhanced by the DSS exposure. This evidence concerns the gene ACTA1 and Hepatic fibrosis.