We found that the protein expression of CYP7A1 and CYP8B1 of BBR treated mice were all significantly decreased in comparison to untreated NASH mice, whereas the expression of liver FXR and SHP was not statistically different between BBR treated mice and untreated NASH mice (Figure 4D; Supplementary Figure S2). This evidence concerns the gene CYP7A1 and metabolic dysfunction-associated steatohepatitis.