Given that NKCC1 has been implicated in [Ca2+]i overload during reperfusion following oxygen-glucose deprivation (X. Chen et al., 2008), we examined the effects of the NKCC1 inhibitors, bumetanide and ethacrynic acid, on the [Ca2+]i burden produced by simultaneous ischemia and acidosis in neurons. The gene discussed is SLC12A2; the disease is ischemia.