The expression of Treg-related genes involving Treg-suppressive function was largely unchanged between CD177+ and CD177− TI Treg cells in human breast cancer (Supplementary Fig. 8f) or ccRCC (Supplementary Fig. 8g), with slight elevation of ICOS, TIGIT, and CTLA4 (Supplementary Fig. 8f, g). Here, ICOS is linked to breast cancer.