Using our newly developed ccRCC single cell RNA sequencing dataset including 13,433 PB and 12,239 TI cells, we identified a cluster of Treg cells based on the expression of FOXP3 and CD25 (IL2RA) (Fig. 1a, b), containing 160 PB and 574 TI Treg cells. This evidence concerns the gene FOXP3 and nonpapillary renal cell carcinoma.