ICOS and breast carcinoma: The expression of Treg-related genes involving Treg-suppressive function was largely unchanged between CD177+ and CD177− TI Treg cells in human breast cancer (Supplementary Fig. 8f) or ccRCC (Supplementary Fig. 8g), with slight elevation of ICOS, TIGIT, and CTLA4 (Supplementary Fig. 8f, g).