In addition, HMGB1 promotes the resistance of osteosarcoma to chemotherapy in vitro, and overexpression of miR-22, which targets the 3′ untranslated region (UTR) of HMGB1, inhibits HMGB1 function and reverses the resistance of tumor cells to chemotherapy [55]. The gene discussed is HMGB1; the disease is osteosarcoma.