ALB and prostate carcinoma: Following successful isolation of EVs from cell‐conditioned media by SEC we used the same approach to isolate EVs from 1 ml of healthy donor serum (spiked with 100 μg of prostate cancer cell, Du145‐derived EVs) showing successful separation of EVs from >92% of the bulk soluble protein, represented by human serum albumin (HSA) (Supplemental Figure S1B).