To assess whether hypoxia results in similar homeostatic increases in antioxidant protein expression, we exposed human neuroblastoma (SH-SY5Y) cells to hypoxia for 8 hours and measured protein expression levels of peroxisomal, cytosolic and mitochondrial antioxidant enzymes including catalase, glutathione peroxidase-1 (GPX1), glutathione peroxidase-4 (GPX4), MnSOD, and Peroxiredoxin-3 (PRDX3) (Figure 2A). This evidence concerns the gene GPX4 and neuroblastoma.