We previously generated a high‐content imaging dataset of control and ALS‐related VCP‐mutant iPSC‐derived MN cultures co‐labelled with a combination of three fluorescent markers, specifically, (i) a nuclear‐specific marker (DAPI), (ii) a neuron‐specific marker of the neurites (β III‐tubulin) and (iii) an antibody against one of five ALS‐relevant RBPs: TDP‐43, SPFQ, FUS, heterogeneous nuclear ribonucleoprotein A1 (hnRNPA1) or heterogeneous nuclear ribonucleoprotein K (hnRNPK).16 Here, FUS is linked to amyotrophic lateral sclerosis.