We next specifically demonstrate the utility of this approach by testing the following hypotheses: (1) ALS‐causing VCP mutations result in previously unrecognised phenotypes contained within the information content of DAPI and/orβ III‐tubulin fluorescence images alone; (2) addition of ALS‐related RBPs immunofluorescence images will improve phenotype detection in an RBP‐specific manner; and (3) conventional extrinsic stressors can recapitulate phenotypic aspects of ALS. This evidence concerns the gene VCP and amyotrophic lateral sclerosis.