A number of T. gondii excretory secretory antigens have been tested as DNA vaccine candidates (e.g. TgGRA24, TgGRA25, TgROP5, TgROP18, and TgDOC2C, etc.)and they showed effective immunity against T. gondii infection, with inductive Th1 type and CD8+ cytotoxic T-lymphocyte responses, and prolonged survival time in mice after infection with T. gondii (Zhang et al., 2018; Xu et al., 2019; Zhu et al., 2020)., Some microneme proteins (MICs) have also been shown to have immunogenic capabilities (Dodangeh et al., 2019). The gene discussed is CD8A; the disease is infection.