Meanwhile, GSEA analysis was performed, revealing that C5AR2 was widely involved in metabolic pathways and biosynthesis in pan-cancer, including TNFα signaling via NFκB, IL6 JAK STAT3 signaling, IL2 STAT5 signaling, inflammatory response, KRAS signaling up, reactive oxygen species pathway, p53 pathway, and apoptosis. This evidence concerns the gene NFKB1 and cancer.