In the present study, we not only confirmed their involvement in the glycolysis process of PCa through bioinformatics but also demonstrated that interfering with KIF20A and PGM2L1 expression could affect glucose consumption and that interfering with HMMR, KIF20A, and GPR87 expression could regulate lactic acid production in vitro. The gene discussed is PGM2L1; the disease is posterior cortical atrophy.