In summary, E. coli inhibited mitophagy and enhanced NLRP3 inflammasome activation and apoptosis, both in vivo and vitro. LGR-1 pretreatment induced PINK1/Parkin-mediated mitophagy, cleared the damaged mitochondria, and reduced ROS production, NLRP3 inflammasome activation and apoptosis under E. coli infection. This evidence concerns the gene NLRP3 and escherichia coli infection.