We observed accurate identification of T cell killed M257-A2 tumor cells over the range of CTL dilution, from 1:1 to 1:100,000, with AUC values consistently > 0.95 for NY-ESO-1 TCR-transduced CD8+ T cell killing of M257-A2 melanoma target tumor cells (Fig. 5b,c). The gene discussed is CD8A; the disease is neoplasm.