To further determine the specific expression of tumor-derived and host-derived CCL2, we isolated CD45− cells, CD45+ immune cells, CD11b+ F4/80+Ly6G− TAMs, and CD11b+ F480- Ly6G+ Ly6C-/low G-MDSCs from the tumor tissue of Hepa1-6 tumor-bearing mice. The gene discussed is ITGAM; the disease is neoplasm.