Subsequently, IF staining and EdU assays were adopted to assess the cell proliferation of CRC cells upon RAB22A silencing, and the results showed that both the Ki-67 positive cells and EdU positive cells were reduced by down-regulation of RAB22A, indicating that RAB22A knockdown could effectively suppress CRC cell proliferation (Fig. 4K-L & Supplementary Fig. 2B-C). This evidence concerns the gene MKI67 and colorectal carcinoma.