Results from IF and EdU assays revealed that the Ki-67 positive cells and EdU stained positive cells were obviously decreased by sh-MCF2L-AS1#1 and sh-MCF2L-AS1#2 compared with those in sh-NC groups, which meant that MCF2L-AS1 silencing repressed cell proliferation in CRC (Fig. 1D-E & Supplementary Fig. 1). Here, MKI67 is linked to colorectal carcinoma.