NEIL3 and Familial prostate cancer: To explore the effect of NEIL3 on prostate cancer, DU145 and PC3 cell lines were transfected with 2 small interfering RNAs to construct a NEIL3 knockdown cell line (Supplementary Figure S1A) or infected with the corresponding packaged lentivirus to establish a stable NEIL3-overexpressing cell line (Supplementary Figure S1B).