Although the effectiveness of DOX for treating GBM is largely impacted by its inefficiency in BBB penetration, it has been shown in vitro that DOX can effectively induce death of GBM cells and DOX‐coated nanoparticles can penetrate a model of BBB composed by a monolayer of Madin–Darby canine kidney transfected with multidrug resistant protein 1.14 The gene discussed is ABCB1; the disease is glioblastoma.