After administration of B. longum 420, B. longum 2012 (a recombinant Bifidobacterium longum displaying galacto-N-biose/lacto-N-biose I binding protein [GL-BP] anchor protein without a WT1 protein), or phosphate-buffered saline (PBS), splenocytes and lymphocytes were collected, cultured, and stimulated with mitomycin-C-treated MBT-2 cells, a mouse bladder cancer cell line, or Db126 or Mp235 peptides,30 which are known WT1-specific CD8 epitopes for both human and mouse. The gene discussed is WT1; the disease is urinary bladder carcinoma.