Corroborating this observation, we demonstrated that neither endogenous SPOP depletion by short hairpin RNAs (shRNAs) nor F102C and F133V mutant expression had any obvious effect on DNMT1, DNMT3A and DNMT3B protein expression in both 22Rv1 and DU145 PCa cell lines (Supplementary Fig. 1g, h). The gene discussed is DNMT3A; the disease is posterior cortical atrophy.