Herein, we confirmed that nmMYLK promotes HCC cell proliferation by activating NF-κB signaling and upregulating its downstream genes Cyclin D1, cIAP1, ICAM-1, NFATC1, EHD1, and SDC4 that are correlated with the malignant phenotype of some kinds of tumors. This evidence concerns the gene NFATC1 and hepatocellular carcinoma.