To examine whether treatment with Bis(7)-tacrine suppresses PCa androgen-independent (AI) cell proliferation, INMT-KD and control Myc-CaP cells cultured in charcoal-treated medium were treated with different concentrations of Bis(7)-tacrine for 3 days and the cell proliferation rates were examined by MTT assay. This evidence concerns the gene MYC and posterior cortical atrophy.