By contrast, high multiplicity infection of nTERT and KO cells with vaccinia virus (VACV) expressing A5-GFP revealed that VACV protein expression was unaffected by the absence of nectin1 (Fig 2E), a result that was corroborated by fluorescence microscopy of VACV infected cells where all nTERT and KO cells were shown to be A5-GFP positive (Fig 2F). Here, NECTIN1 is linked to infection.