The forest map revealed that CDK1 expression was significantly up‐regulated in ESCC (SMD = 1.41; 95% CI 1.00–1.83; Fig. 4A), the AUC was 0.886 (Fig. 4B), and the sensitivity and specificity of combination were 0.760 (95% CI: 0.73–0.79) and 0.900 (95% CI: 0.88–0.91), respectively (Fig. 4C,D). The gene discussed is CDK1; the disease is esophageal squamous cell carcinoma.