Western blotting analysis of CSC and non‐CSC populations of ESCC cells further confirmed that TRIB2 and phosphorylated HDAC2 (S394) levels were significantly elevated, while METTL14 and p21 levels were markedly decreased in CSCs of ESCC cells (Figure 6O). The gene discussed is HDAC2; the disease is esophageal squamous cell carcinoma.