ISH also showed that the abundance of miR‐99a‐5p was low in most paraffin‐embedded ESCC tissues detected (Figure 1H); qPCR analysis confirmed the inverse relationship between miR‐99a‐5p and CD90 in ESCC tissues in Cohort I. High miR‐99a‐5p expression was shown to significantly associate with favorable overall survival (OS) in ESCC patients, which was consistent with TCGA (Figure 1I,J). This evidence concerns the gene THY1 and esophageal squamous cell carcinoma.