Co-culturing CRC cell lines (LS174T and T84) directly with intestinal myofibroblasts (CCD-18Co) in the presence of SCFAs (0.025–4.0 mM) as well PGE1or PGE2 (0.01–100 ng/ml) for 24 hours led to increased expression of MUC2 as compared to a monolayer cell culture system.78 This evidence concerns the gene MUC2 and colorectal carcinoma.