In this work, CDK1 was demonstrated to be a downstream target of miR-195-3p, and it was also revealed that miR-195-3p/CDK1 axis regulated the radiosensitivity of NPC cells, and PCNA, Cyclin D1 and c-Myc expression, which partly explains the mechanism of miR-195-3p in the raidoresistance of NPC cells. The gene discussed is CDK1; the disease is nasopharyngeal carcinoma.