These observations indicated that ABC treatment of CD4+ T cell-depleted B*57:01-Tg/PD-1−/− mice induced skin hypersensitivity mimicking AHS in humans with the significantly elevated cytotoxic effector memory CD8+ T cells in the LN, which might lead to their infiltration into the dermic layer. This evidence concerns the gene CD8A and mitochondrial DNA depletion syndrome 4a.