Further support for increased ROS levels as relevant driver of cellular effects caused by HOTAIRM1 knock-down was obtained by treatment of LN-229 and LN-18 glioma cells with N-acetyl cysteine (NAC), a ROS scavenger, which showed that NAC treatment rescued the decrease in colony formation caused by HOTAIRM1 knock-down (Fig. 3C, Supplementary Fig. 5). This evidence concerns the gene HOTAIRM1 and central nervous system cancer.