Beyond this, through categorizing the expression of RNF144A-AS1 as high or low group by using the mean expression level as the cut-off value (n = 26 > mean; n = 34 ≤ mean) in above 60 paired GC tissues, remarkable associations of RNF144A-AS1 with later-stage diseases were discerned, especially with lymph node metastasis (LNM) (P = 0.015) (Additional file 3: Table S3). Here, RNF144A is linked to metastatic malignant neoplasm in the lymph nodes.