In addition, numerous breast cancer cells were vacuolated after treatment with aqueous extracts of O. majorana, which could induce cell death by apoptosis in MCF7 cells, as evidenced by mitochondrial activity, glucose uptake inhibition, expanded the activity of caspase-7, apoptotic genes, and oxidative stress, intracellular ROS accumulation (Baranauskaite et al., 2017). This evidence concerns the gene CASP7 and breast cancer.