Diosmin performs low to medium cytotoxic properties through inhibition of the cell cycle, induce senescence, apoptosis, and stimulate oxidative stress in breast cancer cells, namely MCF-7 (ER+, PR+/-, HER2-), MDA-MB-231 (ER-, PR-, HER2-), and SK-BR-3 (ER-, PR-, HER2+) (Lewinska et al., 2017; Meiyanto et al., 2012; Utomo et al., 2020). Here, ESR1 is linked to breast cancer.