Finally, we determined the differences in the cell proliferation rate, autophagy-related gene expression, and LC3 and p62 protein expression in radiation-induced thyroid carcinoma tissues between 4W and 7M rats following irradiation via immunohistochemistry, immunofluorescence, and RT2 profiler polymerase chain reaction (PCR) arrays. The gene discussed is SQSTM1; the disease is thyroid gland carcinoma.