Consistent with the roles of ALDH3A1 in converting lipid peroxidation product (4-HNE) into fatty acids [31] (Fig. 4d), immunostaining assay indicated upregulation of ALDH3A1 and downregulation of 4-HNE in NB cells stably over-expressing ecircCUX, which was rescued by silencing of ZRF1 or BRD4 (Additional file 1: Fig. S8a). This evidence concerns the gene ALDH3A1 and neuroblastoma.