Interestingly, whereas all four putative CD4 T cell epitopes sensitized for comparable IFN-γ recall responses when pulsed onto SFM-DCs, there were great differences in therapeutic activity observed when SFM-DCs pulsed with each individual peptide were compared for activity in a therapy model for established rHER-2pos breast cancer using orthotopically implanted TUBO breast carcinoma cells (Figure 5B). Here, CD4 is linked to breast cancer.